General Cell Based Assays
Indigo Biosciences Human Granulocyte Colony-Stimulating Factor Receptor (G-CSFR) All-inclusive Cell-Based Luciferase Reporter Assay Kit, 384-well assay plate format
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO’s Human Granulocyte Colony-Stimulating Factor Receptor reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against G-CSFR. Kit includes cryopreserved reporter cells engineered to provide constitutive expression of the receptor, optimized media for use during cell thawing and in diluting test samples, reference agonist, Luciferase Detection Reagent, cell culture-ready assay plate, and detailed assay protocol. INDIGO’s unique Cryomite™ process provides exceptional cell viability post-thaw and INDIGO’s strict QA process means consistent results lot to lot (product quality data available upon request). This kit allows researchers to achieve superior sensitivity, clear, reproducible results, and eliminate weeks of cell culture work.
Indigo Biosciences Human Granulocyte Colony-Stimulating Factor Receptor (G-CSFR) All-inclusive Cell-Based Luciferase Reporter Assay Kit, 96-well assay plate format
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO’s Human Granulocyte Colony-Stimulating Factor Receptor reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against G-CSFR. Kit includes cryopreserved reporter cells engineered to provide constitutive expression of the receptor, optimized media for use during cell thawing and in diluting test samples, reference agonist, Luciferase Detection Reagent, cell culture-ready assay plate, and detailed assay protocol. INDIGO’s unique Cryomite™ process provides exceptional cell viability post-thaw and INDIGO’s strict QA process means consistent results lot to lot (product quality data available upon request). This kit allows researchers to achieve superior sensitivity, clear, reproducible results, and eliminate weeks of cell culture work.
Bioassay Systems EnzyLight™ ATP Assay Kit. For rapid, quantitative, bioluminescent determination of ATP and evaluation of drug effects on ATP metabolism.
For rapid, quantitative, bioluminescent determination of ATP and evaluation of drug effects on ATP metabolism. Key Features: Safe. Non-radioactive assay. Sensitive and accurate. As low as 0.02 µM ATP or a single cell can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: Z factors of > 0.5 are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems. Method: Luminescence. Samples: Cells etc. Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 0.02 µM.
Indigo Biosciences Reporter Assay System, Human AVPR1A, 384-well
This all-inclusive cell-based luciferase reporter assay kit targets the Human Arginine Vasopressin Receptor 1A (AVPR1A). It uses proprietary mammalian cells engineered for constitutive expression of AVPR1A. The kit includes reporter cells, optimized media, a reference agonist, luciferase detection reagent, and a cell culture-ready assay plate for screening test samples to quantify agonist or antagonist functional activity.
- Clear, reproducible results
- All-inclusive assay systems
- Exceptional cell viability post-thaw
- Consistent results lot to lot
- Utilizes proprietary human cells engineered for constitutive expression of the Human Arginine Vasopressin Receptor 1A (AVPR1A)
- Reporter cells are cryopreserved, allowing immediate dispensing without intermediate treatment steps
Indigo Biosciences INDIGO Biosciences Assay, Tropomysin Receptor Kinase C (TrkC; NTRK3), Human, 384-Well Format
This cell-based reporter assay is designed for the detection of Tropomysin Receptor Kinase C activity. It provides a complete system for screening and evaluating compounds that modulate TrkC receptor signaling.
- Designed for human TrkC.
- Provided in a convenient 384-well assay format.
- Offers reliable and reproducible results for in vitro toxicology solutions.
- Accelerates scientific decision-making in nuclear receptor and in vitro toxicology research.
Indigo Biosciences INDIGO BIOSCIENCES INC Human TrkA Reporter Assay Kit, 384-well Format, Human
This all-inclusive cell-based luciferase reporter assay kit targets Human Tropomyosin receptor kinases A (TrkA/NTRK1). It uses proprietary mammalian cells engineered for constitutive TrkA expression. The kit includes TrkA Reporter Cells, optimized media, a reference activator, Luciferase Detection Reagent, and a cell culture-ready assay plate. It quantifies functional activity (activating or inhibitory) against human TrkA or the coupled Ca+2·calcineurin / NFAT pathway. Provides clear, reproducible results, exceptional cell viability post-thaw, and consistent lot-to-lot results. Store at –80°C; do not refreeze or maintain reporter cells in extended culture.
- Clear, reproducible results
- All-inclusive assay systems
- Exceptional cell viability post-thaw
- Consistent results lot to lot
Bioassay Systems QuantiFluo™ Ammonia Assay Kit. For quantitative determination of ammonia/ammonium concentration by fluorimetry at 360nm/450nm (compatible on handheld fluorimeter catalog# FL360450).
For quantitative determination of ammonia/ammonium and evaluation of drug effects on their metabolism. Key Features: Fast and sensitive. Linear detection range of 0.012 - 1 mM ammonia. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL360/450nm. Samples: Biological (eg urine) and environ-mental samples. Species: All. Procedure: Assay takes 15 min. Kit size: 200 tests. Detection limit: 12 µM.
Bioassay Systems QuantiChrom™ Calcium Assay Kit. For quantitative determination of calcium ion Ca2+ and evaluation of drug effects on calcium metabolism.
For quantitative determination of calcium ion Ca2+ and evaluation of drug effects on calcium metabolism. Key Features: Sensitive and accurate. Use as little as 5 µL samples. Linear detection range 0.08 mg/dL (20 µM) to 20 mg/dL (5 mM) Ca2+ in 96-well plate assay. Simple and high-throughput. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid, protein and minerals such as magnesium, iron and zinc. Method: OD612nm. Samples: Biological, food and environment. Species: All. Procedure: Assay takes 3 min. Kit size: 500 tests. Detection limit: 0.08 mg/dL (20 µM).
Bioassay Systems QuantiChrom™ Uric Acid Assay Kit. For quantitative determination of uric acid and evaluation of drug effects on uric acid metabolism.
For quantitative determination of uric acid and evaluation of drug effects on uric acid metabolism. Key Features: Sensitive and accurate. Use 5 µL samples. Linear detection range 0.22 mg/dL (13 µM) to 30 mg/dL (1785 µM) uric acid in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 30 min. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on serum samples. Method: OD590nm. Samples: Serum, plasma, urine and other biological samples. Species: All. Procedure: Assay takes 30 min. Kit size: 250 tests. Detection limit: 0.2 mg/dL (13 µM).
